I started PhD in fall of 2019. And I am about to defend on 30th. Also, I skipped masters thinking I would gain some years. I am 31 btw...
The COVID happened, bla, bla, we all know that.
I was without a lab for close to 2 years, so, if one wants to get technical, it is more 5 years than 7 years.
I am dealing with this GPCR deorphanization resrarch, of a freaking receptor that has no experimental or computational structure, no jnow G-protein coupling, no known similar receptro other than itself in other species, no known ligand of any kind. At the time I started researching it, there were only 5 papers available, and they all mentioned it in passing. Alone, so, if there will ever be a paper in the next decade, it will be me an my PI, and we all know if there are two names in a paper, the last name rarely does any sh*t.
The back story is, I got handed this research idea over from a supposedly senior PhD. Not really senior but it was his second PhD. And sadly, my advisor show serious partialities - it suffices to say I am at the bottom of the food chain in the lab. So, for about three years, everything I said was vetoed by the guy I got handed over from, and she juat agreed with him. That is where I will leave this.
I applied for a national grant with this research, according to my PI's. Donxt get me wrong, I wrote a currently 4 million something budget project myself, but her input consistently jeapordizing what is doable. And yet, I manage to write plausible enough application to get accepted. And I have recebtly completed the final report, and she became the poster girl, sending me this file about 'success storyx of her details and my project, but wanting me to adjuat the info and the data figures... And she did not even bother to tell me that the results were accepted. My analyeses at the time was not as rigorous as my thesis, but the numbers were the same back then as well, so for the curious ones, I did not coesmetically change my data. The ambiguity and the ridiculous standard deviations are visible from the results. Oh, did I mention she also did not spend much money from my grant to my project, I even recently had to pay 30,000 something from my pocket to progress... The only thing I managed to make it work arrived at my hand exatly 16 months ago as well.
My current, more rigorous results just gave me additional statistics on just how ambiguous my output is. There is a reproducible -barely- trend, that is for sure. But bc my dose response curve calculations in GraphPad does not automatically generate the parameter values at 95% CI, I should not be reporting any IC50 values.
Honestly, it is not even the failure of the project bc hey, sometimes a science does not science. I have ideas on how to support my current results for a paper.
My problem is, I spent 7 years, on something that could have finished in 5 at max. Luckily, moslty bc she did not pay attention, my thesis is to my liking in theory. Oh, did I mention I atopped trying to do some of the things she asked me and took a shorcut to make some things work? Anyways, moving on. I actually delived most of my specific aims. The only three paper relevant to the function of this particular receptor - one fully bioinformatics and two in vivo - have been available since late 2023, and only one can partially serve half of one of my aims, if I had reliable IC50.
I got distracted too much, apologies. The problem is I spent 7 years, with no other papers. Well, that is reaching. So, the summary is, there was another PhD graduated right around the time I started, she webt abroad for a deorphanization, in a pest - not a mammalian receptor, and there was a masters at the time in the lab, she continued her work, and she published in Nat. Comm. as first name - which is good for her, she is a hardworker. She then receive this colab offer from the researcher she collaborated with for her master's research, again another pest. And seeing my research is not taking me anywhere, I suspect she pitied my situation, to include me in the paper, and that has been submitted to a Q1. So, the only paper is that, and that is if it gets accepted.
I suppose another huge problem in freaking academia is, why on earth we can not publosh negative results? Like, I did something and it did not work, so, do not wats etime doing this bc it does not work.
I refuse to remove outliers or apply lenient constraints to my data. Bc if I manage to stay in academia, I do not want my qualifications to be questioned in the future.
But that leaves me, basically with nothing to be questioned for.
Apologies for the rant that is all over the place, my mother has health issues so I can not fully vent to her. The peraon in the lab I can vent to whereas can listen, does not understand bc of her publication - we also suspect some of the paper's success is bc it is a collaboration - it has in silico, in vitro, and in vivo. And she did work with a natural ligand and G-protein coupling established receptor. She actually can publish another paper from that research, but her hands are full so she does not want that at the moment. My closest friend has not stayed in academia, so, whereas he can listen to, he does not relate.
The difficulty of the OhD did not break me, really. A researcher's job is to research. But the stubbornness and stupudity I faced did. Bc I wasted 7 years, and have nothing to show to anyone. The more fruestrating part is, if she asks me to puclish it half assed, like, the in silico part only, I had that data completed for 2 years now. I could have finished this a lot earlier. And would have faced less chances to get scooped. Though, no sane person would study this receptor. Let me tell you this, when I began modeling this protein, AlphaFold was not a thing. AI did not exist.
Thank you, if you have read thua far.
I realize some will think I am exaggerating. It is alright, novody can be fully unbiases. There probably are some aspects I am. And it probably does not help my psyche that I am a week away from my defense. Oh, did I me tion that pest paper suvmitted to a journal, I was dealing with it's revisions and additional analyses until like 2 days ago, so, I am just now writing my thesis. Luckily my progress report were extensuve, and having written the grant final report gave me an outline overall, and outline for in vitro. My lovely asvisor even asked me if the revision will fonish by its due date. And I said, I quit my thesis, solely focusing on the revision...
My point of dramatizing was, I donxt think it is safe for me to give any firther details, as this is a niche are of research, and too much info can give away too much. On that note, I do still think my advisor is the only workable one in my university. So, the sad reality is, I do not regret choosing her per se. I probably would atill chose her, bc I think she is the best z of the worst. I do not regret my work as well, I am doing something I love. Now, choosing this uni, I might mildly regret that.
I also aplogize for the typos. I have been typing a lot these days.