Please advise and share your own personal experience.

We study an autoimmune disease and have to use human sera to perform immunofluorescence staining on mouse tissues to confirm reactivity. Because sera contain thousands of unwanted IgGs, besides the desired pathogenic IgGs, the noise is obscene. We have tried pretreating the sera with guinea pig liver power to absorb nonspecific IgGs, as suggested by the literature, but the improvement is zero to minimal. Any ideas? TIA!

Hello!

I’m a hobbyist/nerd who would like to better understand how T-cells operate and what types of deficiencies are caused by T-cell dysfunction. What are some of the best resources for learning about this? Open to books/websites/talks, etc!

Thank you!

If you like story driven, action RPGs that are also educational, you might like this. I really want to bring the immune system to life but with a strong educational aspect to it. I don't want to gloss over the details, I want to end up making this something that students might actually use in the future. Learning by experiencing is so much better imo.

As above. Thanks in advance.

They also have lysosomal granules… and pattern recognition receptors (PAMP PRRs). Why? My hunch is that evidently if the blood vessels break pathogens have an easy access point. If the owner has IgGs against whatever enters, then PLTs can physically use their bodies dendritic forms to trap the organism. Additionally I assume if their PRRs touch a pathogen they can degranulate onto it and try to kill it. But what do you think?

China’s regulator, the NMPA, approved satri-cel (satricabtagene autoleucel) from CARsgen for advanced stomach and gastroesophageal-junction cancer - the first CAR-T therapy cleared anywhere for a solid tumor.

Curious to hear what everyone thinks about this!! And when the US and Europe will catch up?

https://www.carsgen.com/en/news/20260622/

Hello everyone! It has been quite some time since I last posted, but here I am! But I do need another review and possibly some insights. A few posts ago, it was pointed out that my diagrams required cytokines, you know, for "cell-to-cell" communication. And so I am currently working on it. I'm asking for brief critiques; any will help. I am also debating whether I should include a node for the barrier epithelium, since it is on the front lines and is usually the initial spark for inflammatory responses. That's all, and I would greatly appreciate any insights!

Hi,
I am trying to establish an immuno histochemistry panel on lung sections where I stain macrophages with mouse anti CD68 Ab, epithelial cells with rabbit Pan cytokeratin. I want to add a neutrophil marker (MPO stain/NE/CD66b) but I am not getting a different host.
Does anyone have any suggestions for which Ab I can use to detect neutrophils that work well with paraffin embedded lung sections?

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Asking for a friend.

(The friend is me.)

Master's graduate looking for fully funded PhD positions in Immunology or AMR outside India. If anyone has leads, tips, lab recommendations, or secret academic cheat codes, please send help.

Payment offered: eternal gratitude and occasional microbiology memes.

Hello! I’m looking for some advice for a pipe dream that I have.

I’ve always wanted to experience living on the west coast (San Francisco/Bay Area specifically) but for personal reasons, I can’t settle down and work any long term jobs there (1 year or more). So I was wondering if there is such a thing as a post grad internship or rotational program that would allow me to move there for a couple of months before I put down any roots at home.

For context right now I’m a part time MS student studying infectious disease and microbiology as well as a full time lab tech in a BSL3 virology lab - I’m still a ways off from graduating, im just trying to consider my future options.

Any advice would be appreciated, but if this is a terrible idea please don’t flame me 🥲

For my thesis in microbiology II I picked the topic above as my field of interest. I found that the foreskin has so many benefits from an immunological perspective. My paper is peer reviewed by an MD named Dr Mary Zhang MD, she was my professor and used to be a Chinese cardiologist.

Just bought the Dynabeads T cell activation beads, and the protocol says to wash them first with the help of a magnet... How important is this step? We do not have the magnet... Anyone try an alternate method of washing and/or skipping washing altogether?

Just got some human recombinant interleukin 2 from peprotech, and looks like it needs to be reconstituted initially in Acetic acid... how critical is this? Could I use something else, like hydrochloric acid or just plain dPBS/water? Anyone try anything different?